Hotspot

trnT-GGU-psbD

LSC · 27044-27550 · 507 bp

Back to Eremogone

Pi 0.0071

MarkerSeek score 55.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 7
pi: 0.0071
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACGGTCTCTACGTAACCAGT	55.0	50.0	58.1	57.8	646-703 bp	1.0	79.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	649-706 bp	1.0	79.7
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.3	57.8	623-680 bp	1.0	78.2
trnT-GGU-psbD_p4	TGGTAGAGTAACGCCATGGT	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.4	57.8	645-702 bp	1.0	76.9
trnT-GGU-psbD_p5	ATGGCCCCTTTAACTCAGTG	ACGGTCTCTACGTAACCAGT	50.0	50.0	57.5	57.8	663-720 bp	1.0	76.2