Hotspot

trnE-UUC-trnT-GGU

LSC · 26747-26971 · 225 bp

Back to Eremogone

Pi 0.0498

MarkerSeek score 89.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 23
pi: 0.0498
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	310-588 bp	1.0	86.5
trnE-UUC-trnT-GGU_p2	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	312-590 bp	1.0	86.4
trnE-UUC-trnT-GGU_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	291-569 bp	1.0	86.1
trnE-UUC-trnT-GGU_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	289-567 bp	1.0	86.1
trnE-UUC-trnT-GGU_p5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	309-587 bp	1.0	85.9