Hotspot

matK-trnK-UUU

LSC · 3415-4069 · 655 bp

Back to Eremogone

Pi 0.00369

MarkerSeek score 62.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 5
pi: 0.00369
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TGTGTTGACAAGAACTTTCTCG	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.1	58.2	728-762 bp	1.0	57.9
matK-trnK-UUU_p2	TGTGTTGACAAGAACTTTCTCG	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.1	58.2	727-761 bp	1.0	57.9
matK-trnK-UUU_p3	GTGTTGACAAGAACTTTCTCGT	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.1	58.2	727-761 bp	1.0	57.9
matK-trnK-UUU_p4	GTGTTGACAAGAACTTTCTCGT	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.1	58.2	726-760 bp	1.0	57.9
matK-trnK-UUU_p5	TGTGTTGACAAGAACTTTCTCG	AGCTATTACGGGGGTTGCTA	40.9	50.0	57.1	58.2	752-786 bp	1.0	57.6