Hotspot

rpoA

LSC · 75700-76719 · 1020 bp

Back to Eremochloa

Pi 0.00327

MarkerSeek score 60.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 5
pi: 0.00327
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGATTGTGCCAAAGATGCAA	ACACCTATGCCGCATAATGG	40.0	50.0	56.2	58.1	1221-1222 bp	1.0	65.3
rpoA_p2	AGATTGTGCCAAAGATGCAA	CGCATAATGGATGTCGACCT	40.0	50.0	56.2	57.8	1211-1212 bp	1.0	64.9
rpoA_p3	AGATTGTGCCAAAGATGCAA	CACCTATGCCGCATAATGGA	40.0	50.0	56.2	57.8	1220-1221 bp	1.0	64.6
rpoA_p4	AGATTGTGCCAAAGATGCAAA	ACACCTATGCCGCATAATGG	38.1	50.0	57.0	58.1	1221-1222 bp	1.0	63.4
rpoA_p5	TGCCAAAGATGCAAAATTCCT	ACACCTATGCCGCATAATGG	38.1	50.0	56.9	58.1	1215-1216 bp	1.0	63.2