Hotspot

ndhH

SSC · 115585-116766 · 1182 bp

Back to Eremochloa

Pi 0.00226

MarkerSeek score 60.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 4
pi: 0.00226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GTAGGAGGGCAAAAATGGGT	TTGCATTTTGATGAGCCTCA	50.0	40.0	57.8	55.9	1369 bp	1.0	61.7
ndhH_p2	GTAGGAGGGCAAAAATGGGT	TTTGCATTTTGATGAGCCTCA	50.0	38.1	57.8	56.7	1370 bp	1.0	59.9
ndhH_p3	GTAGGAGGGCAAAAATGGGT	TGCATTTTGATGAGCCTCAT	50.0	40.0	57.8	55.7	1368 bp	1.0	59.8
ndhH_p4	AGTAGGAGGGCAAAAATGGG	TTGCATTTTGATGAGCCTCA	50.0	40.0	57.5	55.9	1370 bp	1.0	59.3
ndhH_p5	CCCAGTAGGAGGGCAAAAAT	TTGCATTTTGATGAGCCTCA	50.0	40.0	57.5	55.9	1373 bp	1.0	59.3