Hotspot

rps15-trnN-GUU

IRa · 117139-118290 · 1152 bp

Back to Eremochloa

Pi 0.00116

MarkerSeek score 59.50

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1152
variable_sites: 2
pi: 0.00116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	AATGTGAAGCAAGTCTCCGT	ACGACCAGTCAGTTAACAGC	45.0	50.0	57.4	57.8	1296 bp	1.0	74.5
rps15-trnN-GUU_p2	AATGTGAAGCAAGTCTCCGT	AGGATTCGAACCTACGACCA	45.0	50.0	57.4	58.2	1309 bp	1.0	74.5
rps15-trnN-GUU_p3	AATGTGAAGCAAGTCTCCGT	GGATTCGAACCTACGACCAG	45.0	55.0	57.4	57.8	1308 bp	1.0	74.1
rps15-trnN-GUU_p4	AATGTGAAGCAAGTCTCCGT	ACCACTGAGCTACTGAGGAA	45.0	50.0	57.4	57.7	1266 bp	1.0	73.2
rps15-trnN-GUU_p5	AATGTGAAGCAAGTCTCCGT	CCCCAAGTAGGATTCGAACC	45.0	55.0	57.4	57.7	1317 bp	1.0	73.1