Hotspot

rps14-psaB

LSC · 38530-38671 · 142 bp

Back to Eremochloa

Pi 0.00469

MarkerSeek score 60.30

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 1
pi: 0.00469
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	ATTTCTGCCGCTTCTTCTCC	GGATTAGCCCACTTTTCCGT	50.0	50.0	58.3	57.9	263-269 bp	1.0	79.1
rps14-psaB_p2	ATTTCTGCCGCTTCTTCTCC	GGTCGGATTAGCCCACTTTT	50.0	50.0	58.3	57.9	267-273 bp	1.0	79.0
rps14-psaB_p3	TCGCTCAAAGGGGAAACTTT	GGATTAGCCCACTTTTCCGT	45.0	50.0	57.6	57.9	336-342 bp	1.0	77.8
rps14-psaB_p4	TCGCTCAAAGGGGAAACTTT	GGTCGGATTAGCCCACTTTT	45.0	50.0	57.6	57.9	340-346 bp	1.0	77.8
rps14-psaB_p5	TTCGCTCAAAGGGGAAACTT	GGATTAGCCCACTTTTCCGT	45.0	50.0	57.6	57.9	337-343 bp	1.0	77.8