Hotspot

ndhF

SSC · 129659-131890 · 2232 bp

Back to Eranthis

Pi 0.466

MarkerSeek score 77.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 834
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGGGTTGAGTTTTTGGTGGA	CCGAGTGGTACGAGGATTTG	50.0	55.0	58.6	58.1	2984 bp	0.5	54.5
ndhF_p2	CGGGTTGAGTTTTTGGTGGA	CGAGTGGTACGAGGATTTGG	50.0	55.0	58.6	58.1	2983 bp	0.5	54.5
ndhF_p3	TCGGGTTGAGTTTTTGGTGG	CCGAGTGGTACGAGGATTTG	50.0	55.0	58.6	58.1	2985 bp	0.5	54.5
ndhF_p4	TCGGGTTGAGTTTTTGGTGG	CGAGTGGTACGAGGATTTGG	50.0	55.0	58.6	58.1	2984 bp	0.5	54.5
ndhF_p5	CGGGTTGAGTTTTTGGTGGA	GAAACCGTGGCACAGATCTA	50.0	50.0	58.6	57.6	2839 bp	0.5	52.1