Hotspot

ndhA

SSC · 120827-122820 · 1994 bp

Back to Eranthis

Pi 0.479

MarkerSeek score 78.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1994
variable_sites: 773
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCTTCGCCCACTTTTGAAT	CGCCCCAAAGCAATTTGATT	45.0	45.0	58.0	57.9	2745 bp	0.5	59.2
ndhA_p2	ACCTTCGCCCACTTTTGAAT	CTCACCACCCCATAGTTTGG	45.0	55.0	58.0	57.9	2941 bp	0.5	58.9
ndhA_p3	CAGGCTCTGGAAGGAATTCC	CGCCCCAAAGCAATTTGATT	55.0	45.0	58.0	57.9	2858 bp	0.5	58.8
ndhA_p4	CGATCGGAGTGTTTTTCCCT	CGCCCCAAAGCAATTTGATT	50.0	45.0	57.9	57.9	2658 bp	0.5	58.5
ndhA_p5	ACCTTCGCCCACTTTTGAAT	GACCGATATACCTTGCAGCC	45.0	55.0	58.0	58.2	2384 bp	0.5	58.3