Hotspot

ccsA

SSC · 127300-128271 · 972 bp

Back to Eranthis

Pi 0.449

MarkerSeek score 80.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 362
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TTCTCGATGGCTTCTCTTGC	AATCGGTAGACACGCTGTTC	50.0	50.0	58.0	58.0	1802 bp	0.5	59.8
ccsA_p2	TTCTCGATGGCTTCTCTTGC	GAAATCGGTAGACACGCTGT	50.0	50.0	58.0	58.0	1804 bp	0.5	59.8
ccsA_p3	TTCTCGATGGCTTCTCTTGC	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1806 bp	0.5	59.8
ccsA_p4	TTCTCGATGGCTTCTCTTGC	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1805 bp	0.5	59.8
ccsA_p5	TTCTCGATGGCTTCTCTTGC	CACCATCCCACACGACTAAG	50.0	55.0	58.0	58.0	1964 bp	0.5	59.8