Hotspot

ndhD-ccsA

SSC · 127047-127299 · 253 bp

Back to Eranthis

Pi 0.496

MarkerSeek score 77.30

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 253
variable_sites: 119
pi: 0.496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAACGGGGCCATTTTACAA	CTGCAATTGTGGCTTCCATG	45.0	50.0	58.0	58.0	887 bp	0.5	59.9
ndhD-ccsA_p2	TCAACGGGGCCATTTTACAA	TCCTTTGGCGAAATCCAACA	45.0	45.0	58.0	58.0	1191 bp	0.5	59.9
ndhD-ccsA_p3	TCAACGGGGCCATTTTACAA	ATGCAGCTCTTTTGTGTGGA	45.0	45.0	58.0	58.0	1307 bp	0.5	59.9
ndhD-ccsA_p4	TCAACGGGGCCATTTTACAA	TGCAGCTCTTTTGTGTGGAT	45.0	45.0	58.0	58.0	1306 bp	0.5	59.9
ndhD-ccsA_p5	TTCTCGATGGCTTCTCTTGC	CTGCAATTGTGGCTTCCATG	50.0	50.0	58.0	58.0	729 bp	0.5	59.8