Hotspot

trnG-UCC-trnR-UCU

LSC · 10835-11003 · 169 bp

Back to Eranthis

Pi 0.075

MarkerSeek score 81.00

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 12
pi: 0.075
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.0	56.7	287-296 bp	1.0	75.5
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAAACAAATTGCGTCCAA	55.0	40.0	58.1	56.7	290-299 bp	1.0	75.2
trnG-UCC-trnR-UCU_p3	CCCTAGCCTTCCAAGCTAAC	TGGAAACAAATTGCGTCCAA	55.0	40.0	57.7	56.7	291-300 bp	1.0	73.0
trnG-UCC-trnR-UCU_p4	CTAACGATGCGGGTTCGATT	TGGAAACAAATTGCGTCCAA	50.0	40.0	58.4	56.7	276-285 bp	1.0	72.5
trnG-UCC-trnR-UCU_p5	TGACGTCGACTATAACCCCT	TGGAAACAAATTGCGTCCAA	50.0	40.0	57.6	56.7	307-316 bp	1.0	71.9