Hotspot

rpl32-trnL-UAG

SSC · 104518-104856 · 339 bp

Back to Eragrostis

Pi 0.0492

MarkerSeek score 73.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 339
variable_sites: 64
pi: 0.0492
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGTAACGAGCATCCAAAACC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.1	57.9	439-470 bp	0.947368	79.4
rpl32-trnL-UAG_p2	GGTAACGAGCATCCAAAACC	ACTGAGATTGGAGAGCAGGT	50.0	50.0	57.1	58.1	960-991 bp	0.947368	78.6
rpl32-trnL-UAG_p3	GGTAACGAGCATCCAAAACC	GATCCGCACAAAAGAGTTGC	50.0	50.0	57.1	58.0	1018-1049 bp	0.947368	78.5
rpl32-trnL-UAG_p4	CCAAAAGGTTTTTCTGGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	57.0	58.0	1000-1031 bp	0.947368	77.6
rpl32-trnL-UAG_p5	ACCAAAAGGTTTTTCTGGGC	GATCCGCACAAAAGAGTTGC	45.0	50.0	57.0	58.0	1001-1032 bp	0.947368	77.6