Hotspot

ndhF-rpl32

SSC · 103340-104325 · 986 bp

Back to Eragrostis

Pi 0.032

MarkerSeek score 71.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 986
variable_sites: 116
pi: 0.032
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGATTACCCAGGCATATTGA	TGACATCGAAGTACGTTTTT	45.0	35.0	54.5	53.2	961-1053 bp	1.0	52.0
ndhF-rpl32_p2	GGATTACCCAGGCATATTGA	TTGACATCGAAGTACGTTTT	45.0	35.0	54.5	53.2	962-1054 bp	1.0	52.0
ndhF-rpl32_p3	GGATTACCCAGGCATATTGA	TTTGACATCGAAGTACGTTT	45.0	35.0	54.5	53.2	963-1055 bp	1.0	52.0
ndhF-rpl32_p4	GGATTACCCAGGCATATTGA	TTTTGACATCGAAGTACGTT	45.0	35.0	54.5	53.2	964-1056 bp	1.0	52.0
ndhF-rpl32_p5	GGATTACCCAGGCATATTGA	TTTTTGACATCGAAGTACGT	45.0	35.0	54.5	53.2	965-1057 bp	1.0	52.0