Hotspot

atpB-rbcL

LSC · 54105-54903 · 799 bp

Back to Eragrostis

Pi 0.0165

MarkerSeek score 67.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 64
pi: 0.0165
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGATCAATACGCCCTGCA	GCTTGAAATCCAACACCTGC	45.0	50.0	57.9	57.9	894-940 bp	1.0	88.4
atpB-rbcL_p2	TTTGATCAATACGCCCTGCA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	858-904 bp	1.0	88.2
atpB-rbcL_p3	TCAATACGCCCTGCACTTTT	GCTTGAAATCCAACACCTGC	45.0	50.0	58.1	57.9	889-935 bp	0.947368	86.1
atpB-rbcL_p4	TCAATACGCCCTGCACTTTT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	853-899 bp	0.947368	85.9
atpB-rbcL_p5	ATCAATACGCCCTGCACTTT	GCTTGAAATCCAACACCTGC	45.0	50.0	57.9	57.9	890-936 bp	0.947368	85.8