Hotspot

ndhC-trnV-UAC

LSC · 50222-51152 · 931 bp

Back to Eragrostis

Pi 0.0228
MarkerSeek score 67.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
931
variable_sites
80
pi
0.0228
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGGAACA GGTTCGAATCCGTATAGCCC 40.9 55.0 57.8 57.9 911-1015 bp 1.0 72.4
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGGAACA CGGTTCGAATCCGTATAGCC 40.9 55.0 57.8 58.3 912-1016 bp 1.0 70.6
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGGAACA GGTTCGAATCCGTATAGCCC 42.9 55.0 56.3 57.9 910-1014 bp 1.0 68.8
ndhC-trnV-UAC_p4 CATATTCGTGAAGCAGGAACA CGGTTCGAATCCGTATAGCC 42.9 55.0 56.3 58.3 911-1015 bp 1.0 66.9
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGGAAC GGTTCGAATCCGTATAGCCC 42.9 55.0 56.1 57.9 911-1015 bp 1.0 66.6