Hotspot

ycf3-trnS-GGA

LSC · 44312-44882 · 571 bp

Back to Eragrostis

Pi 0.0213

MarkerSeek score 71.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 54
pi: 0.0213
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTTTTCCCCTGAGGTTGTCG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.0	58.2	640-760 bp	1.0	89.1
ycf3-trnS-GGA_p2	TTTTTCCCCTGAGGTTGTCG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	590-710 bp	0.947368	87.1
ycf3-trnS-GGA_p3	TTTTTCCCCTGAGGTTGTCG	AGTTCCAATGCTACGCCTTG	50.0	50.0	58.0	58.5	592-712 bp	1.0	86.3
ycf3-trnS-GGA_p4	TTTTTCCCCTGAGGTTGTCG	CAGTTCCAATGCTACGCCTT	50.0	50.0	58.0	58.5	593-713 bp	1.0	86.3
ycf3-trnS-GGA_p5	TTTTTCCCCTGAGGTTGTCG	TCCAATGCTACGCCTTGAAC	50.0	50.0	58.0	58.6	589-709 bp	0.947368	84.2