Hotspot

psaA-ycf3

LSC · 41684-42324 · 641 bp

Back to Eragrostis

Pi 0.0211

MarkerSeek score 68.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 56
pi: 0.0211
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCAAGTCCTAATAACCCCGC	CAGAGCCGTATGAGGTAGGA	55.0	55.0	58.0	58.0	1472-1512 bp	1.0	85.5
psaA-ycf3_p2	CCAAGTCCTAATAACCCCGC	TCAGAGCCGTATGAGGTAGG	55.0	55.0	58.0	58.0	1473-1513 bp	1.0	85.5
psaA-ycf3_p3	GCATCAGCATGTAGGTTCCA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.0	58.0	866-906 bp	0.947368	85.3
psaA-ycf3_p4	CCAAGTCCTAATAACCCCGC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	1325-1365 bp	0.947368	83.7
psaA-ycf3_p5	CTACATCTTGGAACCAGGCC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	1282-1322 bp	0.894737	80.9