Hotspot

atpI-atpH

LSC · 31621-32440 · 820 bp

Back to Eragrostis

Pi 0.035

MarkerSeek score 65.70

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 146
pi: 0.035
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	AATTGGTCGGGTGCTCTTTT	AGAGGGCGAGCATATCTTCT	45.0	50.0	58.0	58.0	969-1701 bp	1.0	91.9
atpI-atpH_p2	AATTGGTCGGGTGCTCTTTT	TTCTGGCTGTCTCGCAATAC	45.0	50.0	58.0	58.0	652-1384 bp	1.0	91.9
atpI-atpH_p3	AGCTTTACTCACATCAGCGG	AGAGGGCGAGCATATCTTCT	50.0	50.0	58.0	58.0	874-1606 bp	1.0	91.8
atpI-atpH_p4	AGCTTTACTCACATCAGCGG	TTCTGGCTGTCTCGCAATAC	50.0	50.0	58.0	58.0	557-1289 bp	1.0	91.8
atpI-atpH_p5	AATTGGTCGGGTGCTCTTTT	AATAAGAGCGCTAGTGCCAC	45.0	50.0	58.0	58.1	747-1479 bp	1.0	91.5