Hotspot

trnD-GUC-psbM

LSC · 15608-16576 · 969 bp

Back to Eragrostis

Pi 0.024

MarkerSeek score 64.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 110
pi: 0.024
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TCAATTGGTTAGAGCACCGC	TGCGAGAATATTTACTTCCA	50.0	35.0	58.6	52.0	826-1077 bp	1.0	52.0
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	TGCGAGAATATTTACTTCCA	55.0	35.0	58.7	52.0	859-1110 bp	1.0	52.0
trnD-GUC-psbM_p3	TCAATTGGTTAGAGCACCGC	ATGCGAGAATATTTACTTCCA	50.0	33.3	58.6	52.7	827-1078 bp	1.0	52.0
trnD-GUC-psbM_p4	TCAATTGGTTAGAGCACCGC	TGCGAGAATATTTACTTCCAT	50.0	33.3	58.6	52.7	826-1077 bp	1.0	52.0
trnD-GUC-psbM_p5	TAGGTATGCCATACACCCCG	TGCGAGAATATTTACTTCCAT	55.0	33.3	58.7	52.7	859-1110 bp	1.0	52.0