Hotspot

petA-psbJ

Genome · 72404-73518 · 1115 bp

Back to Equisetum

Pi 0.0853

MarkerSeek score 76.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1115
variable_sites: 217
pi: 0.0853
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAAGATCCTTCGCGAGTTCA	TTCCATTAATCACAGGCCGG	50.0	50.0	58.0	57.9	1798-1817 bp	1.0	88.0
petA-psbJ_p2	CAAGATCCTTCGCGAGTTCA	GCCAATACGACCGGAAGAAT	50.0	50.0	58.0	58.1	1358-1380 bp	1.0	87.8
petA-psbJ_p3	CGAGGTCGAGGGCAAATTTA	TTCCATTAATCACAGGCCGG	50.0	50.0	58.0	57.9	2074-2093 bp	1.0	87.8
petA-psbJ_p4	CAAGATCCTTCGCGAGTTCA	ACAGGCCGGTTCAATTCATT	50.0	45.0	58.0	58.1	1787-1806 bp	1.0	87.8
petA-psbJ_p5	CGAGGTCGAGGGCAAATTTA	GCCAATACGACCGGAAGAAT	50.0	50.0	58.0	58.1	1634-1656 bp	1.0	87.6