Hotspot

atpB-rbcL

Genome · 64748-65287 · 540 bp

Back to Equisetum

Pi 0.0764

MarkerSeek score 77.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 540
variable_sites: 97
pi: 0.0764
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGACGATAAGGAGCCAACAA	AATCCAACACCTGCTTTGGT	50.0	45.0	58.0	57.9	1051-1096 bp	1.0	89.1
atpB-rbcL_p2	CGACGATAAGGAGCCAACAA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1668-1713 bp	1.0	88.7
atpB-rbcL_p3	CCCGCTCCTCCAAATAATCC	AGAAACGATCTCTCCAACGC	55.0	50.0	58.1	58.0	1701-1746 bp	1.0	87.8
atpB-rbcL_p4	CGACGATAAGGAGCCAACAA	CATGTACCTGCAGTAGCGTT	50.0	50.0	58.0	58.0	1753-1757 bp	0.363636	57.6
atpB-rbcL_p5	CCCGCTCCTCCAAATAATCC	CATGTACCTGCAGTAGCGTT	55.0	50.0	58.1	58.0	1786-1790 bp	0.363636	56.8