Hotspot

psaA-trnS-GGA

Genome · 54640-57054 · 2415 bp

Back to Equisetum

Pi 0.0514

MarkerSeek score 77.20

Primer available yes

Label psaA-trnS-GGA

Feature Metadata

label_name: psaA-trnS-GGA
feature_type: igs
region: Genome
length_bp: 2415
variable_sites: 323
pi: 0.0514
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-trnS-GGA_p1	AATCTTGACTTCCGGTTCCG	ATTCGAACCCTCGGTAAACG	50.0	50.0	57.9	58.0	2501-2635 bp	1.0	89.6
psaA-trnS-GGA_p2	TGACCCACTATTGGCCAAAC	ATTCGAACCCTCGGTAAACG	50.0	50.0	58.1	58.0	2827-2961 bp	1.0	89.4
psaA-trnS-GGA_p3	GTTATTCCAGATGCTCGCCA	CTACACCTTCAACCACTCGG	50.0	55.0	58.0	57.9	2872-2973 bp	0.909091	85.4
psaA-trnS-GGA_p4	GTTATTCCAGATGCTCGCCA	ATTCGAACCCTCGGTAAACG	50.0	50.0	58.0	58.0	2917-3000 bp	0.454545	67.5
psaA-trnS-GGA_p5	TGGTGCAGCTTTGTGGTAAT	CTACACCTTCAACCACTCGG	45.0	55.0	58.0	57.9	2969-2994 bp	0.272727	53.4