Hotspot

tRNA-Asn(GUU)-ndhF

IRb · 115164-115830 · 667 bp

Back to Epipremnum

Pi 0.206

MarkerSeek score 84.40

Primer available yes

Label tRNA-Asn(GUU)-ndhF

Feature Metadata

label_name: tRNA-Asn(GUU)-ndhF
feature_type: igs
region: IRb
length_bp: 667
variable_sites: 130
pi: 0.206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn(GUU)-ndhF_p1	CGGGAGAATTGATGACTGCA	TTTCTTTGCGGGTGAAGGAA	50.0	45.0	58.0	57.9	1142-1535 bp	1.0	90.5
tRNA-Asn(GUU)-ndhF_p2	ACCTGGGGCTTTTTACAAGG	TTTCTTTGCGGGTGAAGGAA	50.0	45.0	58.0	57.9	1069 bp	0.5	59.4
tRNA-Asn(GUU)-ndhF_p3	ACCTGGGGCTTTTTACAAGG	GGGGCGAATCTCGTCTTATC	50.0	55.0	58.0	57.9	1030 bp	0.5	59.4
tRNA-Asn(GUU)-ndhF_p4	ACAGGGAACGGGTTAGGTTA	TTTCTTTGCGGGTGAAGGAA	50.0	45.0	58.0	57.9	955 bp	0.5	59.3
tRNA-Asn(GUU)-ndhF_p5	CGGGAGAATTGATGACTGCA	GGGGCGAATCTCGTCTTATC	50.0	55.0	58.0	57.9	1103 bp	0.5	59.2