Hotspot

tRNA-Leu(CAA)-ndhB

IRb · 99878-100860 · 983 bp

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Pi 0.111
MarkerSeek score 84.00
Primer available yes
Label tRNA-Leu(CAA)-ndhB

Feature Metadata

label_name
tRNA-Leu(CAA)-ndhB
feature_type
igs
region
IRb
length_bp
983
variable_sites
86
pi
0.111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Leu(CAA)-ndhB_p1 GTCTCGCGTGTCTACCATTT TGCAATTGCTCAGGATACCC 50.0 50.0 58.0 57.9 853-1043 bp 1.0 90.7
tRNA-Leu(CAA)-ndhB_p2 CTCGCGTGTCTACCATTTCA TGCAATTGCTCAGGATACCC 50.0 50.0 58.0 57.9 851-1041 bp 1.0 90.7
tRNA-Leu(CAA)-ndhB_p3 GCGTGTCTACCATTTCACCA TGCAATTGCTCAGGATACCC 50.0 50.0 58.2 57.9 848-1038 bp 1.0 89.3
tRNA-Leu(CAA)-ndhB_p4 TCTCGCGTGTCTACCATTTC TGCAATTGCTCAGGATACCC 50.0 50.0 57.7 57.9 852-1042 bp 1.0 88.6
tRNA-Leu(CAA)-ndhB_p5 TCGCGTGTCTACCATTTCAC TGCAATTGCTCAGGATACCC 50.0 50.0 58.3 57.9 850-1040 bp 1.0 88.5