Hotspot

ycf3-tRNA-Ser(GGA)

LSC · 47358-48236 · 879 bp

Back to Epipremnum

Pi 0.117

MarkerSeek score 82.80

Primer available yes

Label ycf3-tRNA-Ser(GGA)

Feature Metadata

label_name: ycf3-tRNA-Ser(GGA)
feature_type: igs
region: LSC
length_bp: 879
variable_sites: 99
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-tRNA-Ser(GGA)_p1	ACACGACAGAAGGCATTGTT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	58.0	58.1	1236-1253 bp	1.0	89.8
ycf3-tRNA-Ser(GGA)_p2	GTCATCCTTCCTGCAAGAGG	CCCTCGGTAAACAAAAGCCT	55.0	50.0	58.0	58.1	1643-1656 bp	1.0	88.1
ycf3-tRNA-Ser(GGA)_p3	TTTCTTTGTCCCTAACGCCC	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.1	58.1	1568-1589 bp	1.0	87.4
ycf3-tRNA-Ser(GGA)_p4	ACACGACAGAAGGCATTGTT	GGACAATTTTGCCCGCTTTT	45.0	45.0	58.0	58.1	1558 bp	0.5	58.8
ycf3-tRNA-Ser(GGA)_p5	GTCATCCTTCCTGCAAGAGG	GGACAATTTTGCCCGCTTTT	55.0	45.0	58.0	58.1	1965 bp	0.5	58.7