Hotspot

tRNA-Glu(UUC)-tRNA-Thr(GGU)

LSC · 33497-34527 · 1031 bp

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Pi 0.176

MarkerSeek score 78.80

Primer available yes

Label tRNA-Glu(UUC)-tRNA-Thr(GGU)

Feature Metadata

label_name: tRNA-Glu(UUC)-tRNA-Thr(GGU)
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 126
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p1	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	785-1095 bp	1.0	90.3
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p2	AGATGTCCTGAACCGCTAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.9	58.1	782-1092 bp	1.0	90.1
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p3	GATGTCCTGAACCGCTAGAC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	784-1094 bp	1.0	89.8
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p4	GATGTCCTGAACCGCTAGAC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	781-1091 bp	1.0	89.6
tRNA-Glu(UUC)-tRNA-Thr(GGU)_p5	GAGATGTCCTGAACCGCTAG	CCATGGCGTTACTCTACCAC	55.0	55.0	57.5	58.1	786-1096 bp	1.0	87.5