Hotspot

tRNA-Lys(UUU)-rps16

LSC · 4383-5049 · 667 bp

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Pi 0.101
MarkerSeek score 82.80
Primer available yes
Label tRNA-Lys(UUU)-rps16

Feature Metadata

label_name
tRNA-Lys(UUU)-rps16
feature_type
igs
region
LSC
length_bp
667
variable_sites
62
pi
0.101
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Lys(UUU)-rps16_p1 CCTTTCAGGATCAGTCGTGG AAAGGAGCTCAACCCACAAG 55.0 50.0 58.0 58.0 860-950 bp 1.0 89.7
tRNA-Lys(UUU)-rps16_p2 CCTTTCAGGATCAGTCGTGG AGCTCAACCCACAAGAACTG 55.0 50.0 58.0 58.0 855-945 bp 1.0 89.6
tRNA-Lys(UUU)-rps16_p3 TAAGAACAGAAGCTCGTCGC AAAGGAGCTCAACCCACAAG 50.0 50.0 58.0 58.0 1069-1169 bp 1.0 89.2
tRNA-Lys(UUU)-rps16_p4 TAAGAACAGAAGCTCGTCGC AGCTCAACCCACAAGAACTG 50.0 50.0 58.0 58.0 1064-1164 bp 1.0 89.2
tRNA-Lys(UUU)-rps16_p5 CCTTTCAGGATCAGTCGTGG AGAGATCTTCGGAGAGTGGG 55.0 55.0 58.0 57.9 944-1034 bp 1.0 89.1