Hotspot

rpl16

LSC · 84430-86016 · 1587 bp

Back to Epipactis

Pi 0.00791

MarkerSeek score 42.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1587
variable_sites: 38
pi: 0.00791
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATTGGGCACTGCTTCTTTGA	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2152-2183 bp	1.0	82.6
rpl16_p2	TGGGCACTGCTTCTTTGATT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2150-2181 bp	1.0	82.6
rpl16_p3	TTGGGCACTGCTTCTTTGAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2151-2182 bp	1.0	82.6
rpl16_p4	CGAGCAATCGCACCAAAAAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	58.0	58.0	2316-2347 bp	1.0	82.2
rpl16_p5	TCTCAATTCCCGAGCAATCG	GCAGGGTTCCCTTACAAACA	50.0	50.0	58.1	58.0	2326-2357 bp	1.0	81.9