Hotspot

rpl32-ndhF

SSC · 129466-130695 · 1230 bp

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Pi 0.0151

MarkerSeek score 60.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1230
variable_sites: 55
pi: 0.0151
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGACATAGAAGTACGCTTTT	GGAGCGGAAAAAGGGGTATT	35.0	50.0	52.3	57.9	1174-1391 bp	1.0	47.4
rpl32-ndhF_p2	TTGACATAGAAGTACGCTTT	GGAGCGGAAAAAGGGGTATT	35.0	50.0	52.3	57.9	1175-1392 bp	1.0	47.4
rpl32-ndhF_p3	TTTGACATAGAAGTACGCTT	GGAGCGGAAAAAGGGGTATT	35.0	50.0	52.3	57.9	1176-1393 bp	1.0	47.4
rpl32-ndhF_p4	TTTTGACATAGAAGTACGCT	GGAGCGGAAAAAGGGGTATT	35.0	50.0	52.3	57.9	1177-1394 bp	1.0	47.4
rpl32-ndhF_p5	TGACATAGAAGTACGCTTTT	ACATAGTAACTGGGAGCGGA	35.0	50.0	52.3	57.8	1186-1403 bp	1.0	47.4