Hotspot

rpl16-rps3

LSC · 86017-86192 · 176 bp

Back to Epipactis

Pi 0.0308

MarkerSeek score 61.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 14
pi: 0.0308
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	GCAGGGTTCCCTTACAAACA	45.0	50.0	57.1	58.0	308-314 bp	1.0	77.2
rpl16-rps3_p2	ACGAGTCACACACTAAGCAT	GCAGGGTTCCCTTACAAACA	45.0	50.0	56.9	58.0	307-313 bp	1.0	75.4
rpl16-rps3_p3	AACGAGTCACACACTAAGCA	CCCTTACAAACAATTCGCGC	45.0	50.0	57.1	58.4	300-306 bp	1.0	74.3
rpl16-rps3_p4	AACGAGTCACACACTAAGCAT	GCAGGGTTCCCTTACAAACA	42.9	50.0	57.6	58.0	308-314 bp	1.0	73.2
rpl16-rps3_p5	AACGAGTCACACACTAAGCA	CCTTACAAACAATTCGCGCT	45.0	45.0	57.1	57.4	299-305 bp	1.0	72.6