Hotspot

psbB-psbT

LSC · 76828-77146 · 319 bp

Back to Epipactis

Pi 0.029

MarkerSeek score 61.10

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 319
variable_sites: 30
pi: 0.029
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	CGGGCTACTTTGAAATCCGA	GGGGCTCAGTACTTCAATGG	50.0	55.0	58.0	58.0	650-696 bp	1.0	87.3
psbB-psbT_p2	GGGATGGAATAGCTGTTGGG	GGGGCTCAGTACTTCAATGG	55.0	55.0	58.0	58.0	952-998 bp	1.0	86.3
psbB-psbT_p3	GGGATGGAATAGCTGTTGGG	GGGGGCTCAGTACTTCAATG	55.0	55.0	58.0	58.0	953-999 bp	1.0	86.3
psbB-psbT_p4	GGGGATGGAATAGCTGTTGG	GGGGCTCAGTACTTCAATGG	55.0	55.0	58.0	58.0	953-999 bp	1.0	86.3
psbB-psbT_p5	GGGGATGGAATAGCTGTTGG	GGGGGCTCAGTACTTCAATG	55.0	55.0	58.0	58.0	954-1000 bp	1.0	86.3