Hotspot

psaJ-rpl33

LSC · 69068-69558 · 491 bp

Back to Epipactis

Pi 0.00604

MarkerSeek score 55.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 491
variable_sites: 9
pi: 0.00604
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	AATGCCCGGCGATTCTTTAT	50.0	45.0	58.1	58.0	659-667 bp	1.0	81.2
psaJ-rpl33_p2	TTGGGTCTTTAGCGGGTCTA	ATGCCCGGCGATTCTTTATT	50.0	45.0	58.1	58.0	658-666 bp	1.0	81.2
psaJ-rpl33_p3	GATGCCTTGTCATTCCCCTT	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.9	58.0	612-620 bp	1.0	80.6
psaJ-rpl33_p4	GATGCCTTGTCATTCCCCTT	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.9	58.0	611-619 bp	1.0	80.6
psaJ-rpl33_p5	TTGGGTCTTTAGCGGGTCTA	ACACCCTTTCGAACACAACA	50.0	45.0	58.1	57.9	637-645 bp	1.0	80.5