Hotspot

psbL-psbF

LSC · 66008-66029 · 22 bp

Back to Epipactis

Pi 0.0503

MarkerSeek score 61.00

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 3
pi: 0.0503
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCAACATTTTGTTCATTCGGGT	TCAGCAATGCAGTTCATCCA	36.4	45.0	57.1	57.8	84 bp	1.0	57.8
psbL-psbF_p2	TCAACATTTTGTTCATTCGGGT	GCAATGCAGTTCATCCAACG	36.4	50.0	57.1	58.4	81 bp	1.0	56.5
psbL-psbF_p3	ACATTTTGTTCATTCGGGTTTGA	TCAGCAATGCAGTTCATCCA	34.8	45.0	57.7	57.8	81 bp	1.0	55.2
psbL-psbF_p4	TCAACATTTTGTTCATTCGGGTT	TCAGCAATGCAGTTCATCCA	34.8	45.0	57.7	57.8	84 bp	1.0	55.1
psbL-psbF_p5	TCAACATTTTGTTCATTCGGGT	GGACTGGCTGTACCTACTGT	36.4	55.0	57.1	58.4	123 bp	1.0	55.0