Hotspot

rps16-trnQ-UUG

LSC · 5835-6778 · 944 bp

Back to Epipactis

Pi 0.0152
MarkerSeek score 59.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
944
variable_sites
38
pi
0.0152
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 999-1041 bp 1.0 85.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 976-1018 bp 1.0 85.1
rps16-trnQ-UUG_p3 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 997-1039 bp 1.0 84.8
rps16-trnQ-UUG_p4 ATCCACAGCCAATCATGTCC CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 1003-1045 bp 1.0 84.5
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 987-1029 bp 1.0 84.5