Hotspot

ndhD

SSC · 119696-121198 · 1503 bp

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Pi 0.00179

MarkerSeek score 40.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 38
pi: 0.00179
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGATTTTTGGGGTTGTCGT	TATGGCATGAGACAACTCGC	45.0	50.0	57.9	58.1	2186-2240 bp	1.0	82.6
ndhD_p2	TGCAAGGCATAAACTCTGCA	TATGGCATGAGACAACTCGC	45.0	50.0	58.1	58.1	2000-2054 bp	1.0	82.6
ndhD_p3	TGGATTTTTGGGGTTGTCGT	TTGGTCCTCTCTCATGAGCA	45.0	50.0	57.9	58.1	2560-2614 bp	1.0	82.4
ndhD_p4	TGCAAGGCATAAACTCTGCA	TTGGTCCTCTCTCATGAGCA	45.0	50.0	58.1	58.1	2374-2428 bp	1.0	82.4
ndhD_p5	TGGATTTTTGGGGTTGTCGT	CGGATTTCCTGAGTGTACGG	45.0	55.0	57.9	58.1	2213-2267 bp	1.0	82.4