Hotspot

ndhD-psaC

SSC · 121199-121333 · 135 bp

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Pi 0.0393

MarkerSeek score 59.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 14
pi: 0.0393
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CGCGAATATCGGCAAAACAA	TATGGCATGAGACAACTCGC	45.0	50.0	57.8	58.1	227-234 bp	1.0	83.5
ndhD-psaC_p2	CGCGAATATCGGCAAAACAA	CGGATTTCCTGAGTGTACGG	45.0	55.0	57.8	58.1	254-261 bp	1.0	82.8
ndhD-psaC_p3	CGCGAATATCGGCAAAACAA	GAAGACTGCGTGGGTTGTAA	45.0	50.0	57.8	58.1	297-304 bp	1.0	81.7
ndhD-psaC_p4	CGCGAATATCGGCAAAACAA	AAGAACAGAAGACTGCGTGG	45.0	50.0	57.8	58.1	304-311 bp	1.0	81.6
ndhD-psaC_p5	CGCGAATATCGGCAAAACAA	CCAAGAACAGAAGACTGCGT	45.0	50.0	57.8	58.1	306-313 bp	1.0	81.6