Hotspot

rpl32-trnL-UAG

SSC · 117606-118366 · 761 bp

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Pi 0.0125

MarkerSeek score 67.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 761
variable_sites: 79
pi: 0.0125
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAGTAATGTAGTAAAGCATTGA	AGCAGCGTGTCTACCAATTT	26.1	45.0	52.1	57.8	767-830 bp	0.958333	50.2
rpl32-trnL-UAG_p2	AAAGTAATGTAGTAAAGCATTGA	GAGCAGCGTGTCTACCAATT	26.1	50.0	52.1	58.3	768-831 bp	0.958333	50.2
rpl32-trnL-UAG_p3	AAAGTAATGTAGTAAAGCATTGA	TCCTAAGAGCAGCGTGTCTA	26.1	50.0	52.1	57.9	774-837 bp	0.958333	50.1
rpl32-trnL-UAG_p4	AAAGTAATGTAGTAAAGCATTGA	CCTAAGAGCAGCGTGTCTAC	26.1	55.0	52.1	57.8	773-836 bp	0.958333	50.1
rpl32-trnL-UAG_p5	AAAGTAATGTAGTAAAGCATTGA	CTAAGAGCAGCGTGTCTACC	26.1	55.0	52.1	57.8	772-835 bp	0.958333	50.1