Hotspot

ndhF-rpl32

SSC · 116765-117443 · 679 bp

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Pi 0.00965

MarkerSeek score 66.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 679
variable_sites: 62
pi: 0.00965
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACTGGGAGTGGAACAAA	TTCTTTTACTGTCGCCCAGC	50.0	50.0	57.6	58.5	781-843 bp	0.958333	82.1
ndhF-rpl32_p2	ACTGGGAGTGGAACAAAAGG	TTCTTTTACTGTCGCCCAGC	50.0	50.0	57.6	58.5	778-840 bp	0.958333	82.1
ndhF-rpl32_p3	CTGGGAGTGGAACAAAAGGT	TTCTTTTACTGTCGCCCAGC	50.0	50.0	57.6	58.5	777-839 bp	0.958333	82.1
ndhF-rpl32_p4	TAGGAACTGGGAGTGGAACA	TTCTTTTACTGTCGCCCAGC	50.0	50.0	57.6	58.5	783-845 bp	0.958333	81.7
ndhF-rpl32_p5	GGAACTGGGAGTGGAACAAA	GCCCAGCACCTTTTTGAATT	50.0	45.0	57.6	57.5	768-830 bp	0.958333	81.7