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psbT-psbN

LSC · 76904-76978 · 75 bp

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Pi 0.0376

MarkerSeek score 60.10

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 11
pi: 0.0376
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GGTTCCGACTAAAAGGGCAA	GATCCATTCGAGGAACACGG	50.0	55.0	58.1	58.4	127 bp	1.0	83.3
psbT-psbN_p2	GGTTCCGACTAAAAGGGCAA	GGGTACGCCTTATATACCGC	50.0	55.0	58.1	57.7	175 bp	1.0	82.4
psbT-psbN_p3	AAGGTTCCGACTAAAAGGGC	GGGTACGCCTTATATACCGC	50.0	55.0	57.8	57.7	177 bp	1.0	81.6
psbT-psbN_p4	GGTTCCGACTAAAAGGGCAA	GTTTTACCGGGTACGCCTTA	50.0	50.0	58.1	57.6	183 bp	1.0	81.4
psbT-psbN_p5	GGTTCCGACTAAAAGGGCAA	TGTCAGTTTTACCGGGTACG	50.0	50.0	58.1	57.6	188 bp	1.0	80.8