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rps12-clpP

LSC · 71262-72447 · 1186 bp

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Pi 0.00667

MarkerSeek score 73.30

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 1186
variable_sites: 78
pi: 0.00667
unique_haplotype_count: 45
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	ATACATGTTCCTCGACGCTG	GACACCCTCGGGAAGTATGA	50.0	55.0	57.8	58.5	901-1391 bp	1.0	83.0
rps12-clpP_p2	ATACATGTTCCTCGACGCTG	TATGTCAGCAGAAGAAGCCC	50.0	50.0	57.8	57.3	953-1443 bp	1.0	81.4
rps12-clpP_p3	ATACATGTTCCTCGACGCTG	ACACCCTCGGGAAGTATGAC	50.0	55.0	57.8	58.8	900-1390 bp	1.0	80.7
rps12-clpP_p4	ATACATGTTCCTCGACGCTG	GTCAGCAGAAGAAGCCCAAG	50.0	55.0	57.8	58.8	950-1440 bp	1.0	80.3
rps12-clpP_p5	TACATGTTCCTCGACGCTGA	GACACCCTCGGGAAGTATGA	50.0	55.0	58.8	58.5	900-1390 bp	1.0	78.0