Hotspot

petA-psbJ

LSC · 65265-65886 · 622 bp

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Pi 0.00738

MarkerSeek score 64.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 37
pi: 0.00738
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCATCACGTGTTCAAGGTC	CTGATAGGTACTGCAACGGG	50.0	55.0	57.8	57.8	819-841 bp	1.0	82.3
petA-psbJ_p2	CCATCACGTGTTCAAGGTCT	CTGATAGGTACTGCAACGGG	50.0	55.0	57.8	57.8	818-840 bp	1.0	82.3
petA-psbJ_p3	TCCATCACGTGTTCAAGGTC	GCTGATAGGTACTGCAACGG	50.0	55.0	57.8	58.4	820-842 bp	1.0	80.7
petA-psbJ_p4	TCCATCACGTGTTCAAGGTC	GGCTGATAGGTACTGCAACG	50.0	55.0	57.8	58.4	821-843 bp	1.0	80.7
petA-psbJ_p5	CCATCACGTGTTCAAGGTCT	GCTGATAGGTACTGCAACGG	50.0	55.0	57.8	58.4	819-841 bp	1.0	80.6