Hotspot

accD-psaI

LSC · 61523-62369 · 847 bp

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Pi 0.00759

MarkerSeek score 66.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 41
pi: 0.00759
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAGGCGTTCTGAGTGAGTTG	ACTAGGCCCACGATAGGTAC	50.0	55.0	58.1	58.0	533-1013 bp	1.0	86.6
accD-psaI_p2	AAGGCGTTCTGAGTGAGTTG	CTAGGCCCACGATAGGTACA	50.0	55.0	58.1	58.0	532-1012 bp	1.0	86.6
accD-psaI_p3	AAGGCGTTCTGAGTGAGTTG	GCAGGAAATACTAGGCCCAC	50.0	55.0	58.1	58.0	542-1022 bp	1.0	86.4
accD-psaI_p4	AAGGCGTTCTGAGTGAGTTG	AGGAAATACTAGGCCCACGA	50.0	50.0	58.1	57.8	540-1020 bp	1.0	85.3
accD-psaI_p5	AAGGCGTTCTGAGTGAGTTG	CAGGAAATACTAGGCCCACG	50.0	55.0	58.1	57.8	541-1021 bp	1.0	84.7