Hotspot

ndhC-trnV-UAC

LSC · 52426-54103 · 1678 bp

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Pi 0.00391

MarkerSeek score 62.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1678
variable_sites: 71
pi: 0.00391
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGCCAAAATTCCGGAAATG	CCGAGAATGTCTACGGTTCG	50.0	55.0	58.0	58.2	1379-1847 bp	1.0	85.5
ndhC-trnV-UAC_p2	CCTTCACTAATTGGGGCCAA	CCGAGAATGTCTACGGTTCG	50.0	55.0	57.8	58.2	1392-1860 bp	1.0	83.9
ndhC-trnV-UAC_p3	CTGGCCCTTCACTAATTGGG	CCGAGAATGTCTACGGTTCG	55.0	55.0	58.2	58.2	1397-1865 bp	1.0	83.8
ndhC-trnV-UAC_p4	GGGCCAAAATTCCGGAAATG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.0	58.7	1364-1832 bp	1.0	81.5
ndhC-trnV-UAC_p5	GGGCCAAAATTCCGGAAATG	CGAGAATGTCTACGGTTCGA	50.0	50.0	58.0	57.2	1378-1846 bp	1.0	80.4