Hotspot

ycf2

IRb · 91767-98486 · 6720 bp

Back to Epilobium

Pi 0.00202

MarkerSeek score 48.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6720
variable_sites: 57
pi: 0.00202
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTTTGAGTTGTTCTGCCGGA	ATCTCCAGGGTCCCAAATGA	45.0	50.0	58.0	58.0	1010-1142 bp	1.0	85.9
ycf2_p2	TTTTGAGTTGTTCTGCCGGA	AATCCATCTCGTGTCTGGGA	45.0	50.0	58.0	58.1	955-1087 bp	1.0	85.1
ycf2_p3	TTTTGAGTTGTTCTGCCGGA	CACTCTGCAAGAACTCCGAA	45.0	50.0	58.0	57.8	924-1056 bp	1.0	85.0
ycf2_p4	TTCGAACTTGGAACGAGCAT	ATCTCCAGGGTCCCAAATGA	45.0	50.0	57.8	58.0	1056-1188 bp	1.0	84.9
ycf2_p5	GGTCCCAGTGTACCAGATCT	ATCTCCAGGGTCCCAAATGA	55.0	50.0	58.1	58.0	1347-1479 bp	1.0	84.7