Hotspot

ccsA-ndhD

SSC · 118995-119423 · 429 bp

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Pi 0.0203

MarkerSeek score 68.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 429
variable_sites: 42
pi: 0.0203
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAATTGTGGCTTTTCTGGGC	TCAGTTGACAAAGTGGAAGCT	50.0	42.9	58.2	57.7	573-589 bp	1.0	78.0
ccsA-ndhD_p2	GCAATTGTGGCTTTTCTGGG	TCAGTTGACAAAGTGGAAGCT	50.0	42.9	58.2	57.7	574-590 bp	1.0	78.0
ccsA-ndhD_p3	ATTGTGGCTTTTCTGGGCTT	TCAGTTGACAAAGTGGAAGCT	45.0	42.9	58.3	57.7	571-587 bp	1.0	77.5
ccsA-ndhD_p4	AATTGTGGCTTTTCTGGGCT	TCAGTTGACAAAGTGGAAGCT	45.0	42.9	58.3	57.7	572-588 bp	1.0	77.4
ccsA-ndhD_p5	TTCCGCAATTGTGGCTTTTC	TCAGTTGACAAAGTGGAAGCT	45.0	42.9	57.9	57.7	578-594 bp	0.933333	75.2