Hotspot

petA-psbJ

LSC · 66664-67661 · 998 bp

Back to Epilobium

Pi 0.00781

MarkerSeek score 68.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 38
pi: 0.00781
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.0	57.8	1180-1230 bp	1.0	83.0
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TACTCCGGGTTAGGCTCATC	50.0	55.0	58.0	58.3	1131-1181 bp	1.0	82.6
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	CGGGTTAGGCTCATCTCTCT	50.0	55.0	58.0	58.3	1126-1176 bp	1.0	82.6
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.8	57.8	1163-1213 bp	1.0	82.1
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.2	57.8	1177-1227 bp	1.0	81.9