Hotspot

ycf4-cemA

LSC · 63951-64822 · 872 bp

Back to Epilobium

Pi 0.00793

MarkerSeek score 70.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 39
pi: 0.00793
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	TAAGAGGGAGATCCACCAGG	50.0	55.0	58.0	57.5	1012-1103 bp	1.0	80.7
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	TAAGAGGGAGATCCACCAGG	50.0	55.0	58.1	57.5	966-1057 bp	1.0	80.0
ycf4-cemA_p3	CCTTGACGCGTAGTGATGAT	TAAGAGGGAGATCCACCAGG	50.0	55.0	57.8	57.5	1036-1127 bp	1.0	79.0
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	TAAGAGGGAGATCCACCAGG	45.0	55.0	57.7	57.5	969-1060 bp	1.0	78.4
ycf4-cemA_p5	GCGCGTACCAATTGAAGTTT	TAAGAGGGAGATCCACCAGG	45.0	55.0	57.7	57.5	963-1054 bp	1.0	78.4