Hotspot

trnG-GCC-trnM-CAU

LSC · 38424-38604 · 181 bp

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Pi 0.0165

MarkerSeek score 69.50

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 14
pi: 0.0165
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	271-316 bp	1.0	84.9
trnG-GCC-trnM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	270-315 bp	1.0	84.9
trnG-GCC-trnM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TATCTTCATCGCGGGGTAGA	50.0	50.0	57.8	57.7	299-344 bp	1.0	84.0
trnG-GCC-trnM-CAU_p4	TCTTTGCCAAGGAGAAGACG	AGGTCATGGGTTCAAATCCC	50.0	50.0	57.8	57.5	246-291 bp	1.0	82.8
trnG-GCC-trnM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TTCTTATCTTCATCGCGGGG	50.0	50.0	57.8	57.5	303-348 bp	1.0	82.0