Hotspot

rpoB-trnC-GCA

LSC · 27829-29097 · 1269 bp

Back to Epilobium

Pi 0.00936

MarkerSeek score 70.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1269
variable_sites: 65
pi: 0.00936
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGACATTCCGCCATTTCCAT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.6	58.1	1322-1376 bp	1.0	83.3
rpoB-trnC-GCA_p2	AGACATTCCGCCATTTCCAT	TTGTTAATCAGGCGACACCC	45.0	50.0	57.6	58.2	1340-1394 bp	1.0	82.4
rpoB-trnC-GCA_p3	GTTCTTCCGTTAAGCCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1395-1449 bp	0.933333	79.5
rpoB-trnC-GCA_p4	AGTTCTTCCGTTAAGCCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1396-1450 bp	0.933333	79.4
rpoB-trnC-GCA_p5	GTTCTTCCGTTAAGCCCTGA	TTGTTAATCAGGCGACACCC	50.0	50.0	57.5	58.2	1413-1467 bp	0.933333	78.7